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αsat  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc αsat
    αsat, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%B1sat/SimpleChIP+Human+alpha+Satellite+Repeat+Primers/pm39422615-262-7-8
    Average 93 stars, based on 25 article reviews
    αsat - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Werner syndrome RECQ helicase participates in and directs maintenance of the protein complexes of constitutive heterochromatin in proliferating human cells
    Article Snippet: ChIP was performed according to the Abcam protocol as described in , with 5μg of chromatin using rabbit α-Histone H3K9me3 Cat. No. A2217P (Diagenode) antibody or the equivalent amount of rabbit IgG Cat. No. C15410206 (Diagenode). .. Input and pulldown DNA was analyzed in triplicates by qPCR with iTaq Universal SYBR Green supermix (Bio-Rad) and the following pairs of primers: LINE1 5’UTR: 5’GATGATGGTGATGTACAGATGGG3’ and 5’AGCCTAACTGGGAGGCACCC3’ ( ) SATII: 5’TCATCGAATGGAAATGAAAGGAGTCATCATCT3’ and 5’CGACCATTGGATGATTGCAGTCAA3’ ( ); αSAT (CST Cat. No. 4486). ..

    SYBR Green Assay:

    Article Title: Werner syndrome RECQ helicase participates in and directs maintenance of the protein complexes of constitutive heterochromatin in proliferating human cells
    Article Snippet: ChIP was performed according to the Abcam protocol as described in , with 5μg of chromatin using rabbit α-Histone H3K9me3 Cat. No. A2217P (Diagenode) antibody or the equivalent amount of rabbit IgG Cat. No. C15410206 (Diagenode). .. Input and pulldown DNA was analyzed in triplicates by qPCR with iTaq Universal SYBR Green supermix (Bio-Rad) and the following pairs of primers: LINE1 5’UTR: 5’GATGATGGTGATGTACAGATGGG3’ and 5’AGCCTAACTGGGAGGCACCC3’ ( ) SATII: 5’TCATCGAATGGAAATGAAAGGAGTCATCATCT3’ and 5’CGACCATTGGATGATTGCAGTCAA3’ ( ); αSAT (CST Cat. No. 4486). ..

    Derivative Assay:

    Article Title: Genome-wide survey of D/E repeats in human proteins uncovers their instability and aids in identifying their role in the chromatin regulator ATAD2
    Article Snippet: Input and pulldown DNA samples were analyzed in triplicates by qPCR with iTaq Universal SYBR Green supermix (Bio-Rad) and the following pairs of primers: Alu ChIP: 5′AATGGTACGATCTCGGCTCA-3′ and 5′TAGCCAGGTGTGGTGACTTG-3′ SATIII ChIP: 5′AATCAACCCGAGTGCAATCNGAATGGAATCG-3′ and 5′TCCATTCCATTCCTGTACTCGG-3′. .. Alu and SATIII primers were described in Shen et al. SATII: 5′TCATCGAATGGAAATGAAAGGAGTCATCATCT-3′ and 5′CGACCATTGGATGATTGCAGTCAA-3′ were derived from the chr.1 SATII sequence, GenBank: X72623.1 . αSAT: (CST Cat. No. 4486). .. RNAs were isolated using RNeasy Plus RNA isolation kit (Qiagen) and additionally purified of DNA contamination using DNA-free kit (Thermo Fisher).

    Sequencing:

    Article Title: Genome-wide survey of D/E repeats in human proteins uncovers their instability and aids in identifying their role in the chromatin regulator ATAD2
    Article Snippet: Input and pulldown DNA samples were analyzed in triplicates by qPCR with iTaq Universal SYBR Green supermix (Bio-Rad) and the following pairs of primers: Alu ChIP: 5′AATGGTACGATCTCGGCTCA-3′ and 5′TAGCCAGGTGTGGTGACTTG-3′ SATIII ChIP: 5′AATCAACCCGAGTGCAATCNGAATGGAATCG-3′ and 5′TCCATTCCATTCCTGTACTCGG-3′. .. Alu and SATIII primers were described in Shen et al. SATII: 5′TCATCGAATGGAAATGAAAGGAGTCATCATCT-3′ and 5′CGACCATTGGATGATTGCAGTCAA-3′ were derived from the chr.1 SATII sequence, GenBank: X72623.1 . αSAT: (CST Cat. No. 4486). .. RNAs were isolated using RNeasy Plus RNA isolation kit (Qiagen) and additionally purified of DNA contamination using DNA-free kit (Thermo Fisher).



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    Journal: iScience

    Article Title: Genome-wide survey of D/E repeats in human proteins uncovers their instability and aids in identifying their role in the chromatin regulator ATAD2

    doi: 10.1016/j.isci.2022.105464

    Figure Lengend Snippet:

    Article Snippet: αSAT primer pair , CST , Cat. No. 4486.

    Techniques: Plasmid Preparation, Recombinant, Protease Inhibitor, Magnetic Beads, Reverse Transcription, Isolation, SYBR Green Assay, Western Blot, Mutagenesis, Cloning, Introduce, Quantitative RT-PCR, Sequencing, Software